The American Journal of Pathology
Volume 178, Issue 5 , Pages 2424-2436 , May 2011

Ultrastructural Analysis Reveals cAMP-Dependent Enhancement of Microvascular Endothelial Barrier Functions via Rac1-Mediated Reorganization of Intercellular Junctions

  • Volker Spindler

      Affiliations

    • Institute of Anatomy and Cell Biology, University of Würzburg, Würzburg, Germany
    • ,
  • Dominik Peter

      Affiliations

    • Institute of Anatomy and Cell Biology, University of Würzburg, Würzburg, Germany
    • ,
  • Gregory S. Harms

      Affiliations

    • Rudolf-Virchow-Center, University of Würzburg, Würzburg, Germany
    • ,
  • Esther Asan

      Affiliations

    • Institute of Anatomy and Cell Biology, University of Würzburg, Würzburg, Germany
    • ,
  • Jens Waschke

      Affiliations

    • Institute of Anatomy and Cell Biology, University of Würzburg, Würzburg, Germany
    • Corresponding Author InformationAddress reprint request to Jens Waschke, M.D., Institute of Anatomy and Cell Biology, University of Würzburg, Koellikerstrasse 6, 97070 Würzburg, Germany

,Accepted 25 January 2011.

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Supplemental Figure S1

Characterization of HDMECs with vascular and lymphatic endothelial cell markers. Left: HDMECs displayed membrane-positive staining for platelet endothelial cell adhesion molecule 1 (PECAM1), angiopoietin receptor Tie-2, and vascular endothelial growth factor receptor 2 (VEGFR2). Right: HDMECs were negative for lymphatic endothelial cell marker prospero homeobox protein 1 (PROX1). Some cells were positive for lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1). Images are representatives of three independent experiments. Scale bar = 20 μm.

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Supplemental Table S1 Other 0 MB

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 Supported by a grant from the Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 688, Teilprojekt A4).

 V.S. and D.P. contributed equally to this work.

 Supplemental material for this article can be found at http://ajp.amjpathol.org or at doi: 10.1016/j.ajpath.2011.01.014.

PII: S0002-9440(11)00123-4

doi: 10.1016/j.ajpath.2011.01.014

The American Journal of Pathology
Volume 178, Issue 5 , Pages 2424-2436 , May 2011

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