Overexpression of Tumor Necrosis Factor-α in the Lungs Alters Immune Response, Matrix Remodeling, and Repair and Maintenance Pathways

Heat maps of microarray gene expression for all KEGG pathways. Heat maps were generated for all KEGG pathways using microarray data for wild-type and SP-C/TNF-α mice (n = 5 per genotype). Red indicates a high level of expression relative to the reference RNA, whereas green indicates low relative expression. The heat maps can be interrogated for genes (using the gene symbol) and pathways (using the KEGG pathway name) of interest using search functions.

Validation of selected microarray probe intensity ratios [SP-C/TNF-α:wild type (WT)] by real-time PCR. Asterisks indicate probes within background for the wild-type samples according to microarray analysis, which likely accounts for the difference in fold change between microarray and PCR approaches.