Department of Physical Therapy and Medicine, University of Tokyo School of Medicine, Hongo, Bunkyo-ku, Tokyo (Japan)
Summary
(1)Sodium [1-14C]acetate was sextensively incorporated into the lipid fractions of chick aortic cells in secondary cultures maintained as monolayers, indicating a linear relation between the incorporation rate and the incubation time over 8 h.
(2)When laminaran sulfate was added to this culture system, at concentrations between 2.5 and 40 μg/ml culture medium for 24–72 h, it depressed lipid synthesis in aortic cells, as measured by [14C]acetate incorporation rates into the lipid fraction.
(3)Inhibition of lipid synthesis depended on the concentration of laminaran sulfate and its incubation time; inhibition was greater in the neutral fat and free sterol fractions than in the phospholipid fraction.
Key words: Atherosclerosis, Cultured aortic cells, Laminaran sulfate, Lipid synthesis, Sulfated polysaccharide
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