The Journal of Molecular Diagnostics
Volume 14, Issue 1 , Pages 30-37 , January 2012

Application of Microfluidic Technology to the BIOMED-2 Protocol for Detection of B-Cell Clonality

  • Alberto Zamò

      Affiliations

    • Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Verona, Italy
    • Corresponding Author InformationAddress reprint requests to Alberto Zamò, M.D., Ph.D., Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Piazzale L.A. Scuro, 10, 37134 Verona, Italy
    • ,
  • Anna Bertolaso

      Affiliations

    • Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Verona, Italy
    • ,
  • Annemiek W.M. van Raaij

      Affiliations

    • Department of Pathology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    • Corresponding Author InformationAddress reprint requests to Alberto Zamò, M.D., Ph.D., Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Piazzale L.A. Scuro, 10, 37134 Verona, Italy
    • ,
  • Francesca Mancini

      Affiliations

    • Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Verona, Italy
    • ,
  • Maria Scardoni

      Affiliations

    • Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Verona, Italy
    • ,
  • Marina Montresor

      Affiliations

    • Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Verona, Italy
    • ,
  • Fabio Menestrina

      Affiliations

    • Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Verona, Italy
    • ,
  • Johan H.J.M. van Krieken

      Affiliations

    • Department of Pathology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    • ,
  • Marco Chilosi

      Affiliations

    • Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Verona, Italy
    • ,
  • Patricia J.T.A. Groenen

      Affiliations

    • Department of Pathology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    • ,
  • Aldo Scarpa

      Affiliations

    • Department of Pathology and Diagnostics, Section of Anatomic Pathology, University of Verona, Verona, Italy

,Accepted 22 July 2011.

Title About Type File Size
Supplemental Figure S1

Bioanalyzer and GeneScan electropherograms of the sensitivity experiments for the IGH tubes (FR1, FR2, and FR3) and IGK tubes (VJ and DE rearrangements) obtained by mixing DNA from a mantle cell lymphoma cell line with DNA from a lymph node-reactive sample. The microfluidic chip-based electrophoresis approach had a sensitivity of 10% for all IGH and IGK tubes. GeneScan analysis had a sensitivity of 1% for IGH tube B (FR2) and tube C (FR3) and a sensitivity of 5% for IGH tube A (FR1) and for IGK tubes A and B.

 Other 0 MB
Supplemental Table S1 Other 0 MB

Please note that add-on components may require plug-in applications.

 Supported by the Italian Association for Cancer Research [Associazione Italiana per la Ricerca sul Cancro (AIRC)] and by Fondazione Cariverona.

 A.Z. and A.B. contributed equally to the present work.

 P.J.T.A.G. and A.S. contributed equally to the present work as senior author.

 Supplemental material for this article can be found at http://jmd.amjpathol.org or at doi: 10.1016/j.jmoldx.2011.07.007.

PII: S1525-1578(11)00258-3

doi: 10.1016/j.jmoldx.2011.07.007

The Journal of Molecular Diagnostics
Volume 14, Issue 1 , Pages 30-37 , January 2012

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