The Journal of Molecular Diagnostics
Volume 14, Issue 1 , Pages 12-21 , January 2012

Influence of RNA Labeling on Expression Profiling of MicroRNAs

  • John S. Kaddis

      Affiliations

    • Departments of Pathology and Laboratory Medicine, Children's Hospital Los Angeles Saban Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California
    • ,
  • Daniel H. Wai

      Affiliations

    • Departments of Pathology and Laboratory Medicine, Children's Hospital Los Angeles Saban Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California
    • ,
  • Jessica Bowers

      Affiliations

    • Genisphere LLC, Hatfield, Pennsylvania
    • ,
  • Nicole Hartmann

      Affiliations

    • Novartis Institutes for BioMedical Research, Novartis, Basel, Switzerland
    • ,
  • Lukas Baeriswyl

      Affiliations

    • Novartis Institutes for BioMedical Research, Novartis, Basel, Switzerland
    • ,
  • Sheetal Bajaj

      Affiliations

    • Departments of Pathology and Laboratory Medicine, Children's Hospital Los Angeles Saban Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California
    • ,
  • Michael J. Anderson

      Affiliations

    • Departments of Pathology and Laboratory Medicine, Children's Hospital Los Angeles Saban Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California
    • ,
  • Robert C. Getts

      Affiliations

    • Genisphere LLC, Hatfield, Pennsylvania
    • ,
  • Timothy J. Triche

      Affiliations

    • Departments of Pathology and Laboratory Medicine, Children's Hospital Los Angeles Saban Research Institute and Keck School of Medicine, University of Southern California, Los Angeles, California
    • USC/CHLA Genome Core Laboratory, Children Hospital Los Angeles, Los Angeles, California
    • Corresponding Author InformationAddress reprint requests to Timothy J. Triche, M.D., Ph.D., Department of Pathology, Children's Hospital Los Angeles, 4650 Sunset Blvd., Los Angeles, CA 90027

,Accepted 22 August 2011.

Title About Type File Size
Supplemental Figure S1

Concordance of detection calls within and between RNA labeling kits. For each input RNA amount (0.1 to 1.0 μg), the number of absent probes are represented by shaded bars, present by black and white checked bars, and discrepant as a black line. Discrepant probes are those with an absent call on one chip and a present call on a corresponding replicate array. Detection calls were generated for 7788 of 7819 probes on the array.

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Supplemental Table S1 Other 0 MB
Supplemental Table S2 Other 0 MB
Supplemental Table S3 Other 0 MB

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 Supported in part by the National Cancer Institute, Strategic Partnering to Evaluate Cancer Signatures (U01CA-114757, to T.J.T.).

 Disclosures: R.C.G. and J.B. are employed by Genisphere LLC and provided the microarrays and corresponding reagents for the RNA titration experiments. N.H. and L.B. are employed by Novartis and provided the microarrays and corresponding reagents for the synthetic spike-in experiments.

 Supplemental material for this article can be found at http://jmd.amjapthol.org or at doi: 10.1016/j.jmoldx.2011.08.005.

PII: S1525-1578(11)00290-X

doi: 10.1016/j.jmoldx.2011.08.005

The Journal of Molecular Diagnostics
Volume 14, Issue 1 , Pages 12-21 , January 2012

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