Progress in Nuclear Magnetic Resonance Spectroscopy
Volume 32, Issue 2 , Pages 107-139, 30 April 1998

Ambiguous NOEs and automated NOE assignment

Structural Biology Programme, European Molecular Biology Laboratory, Meyerhofstr. 1, D-69117 Heidelberg, Germany

Received 30 October 1997

Keywords:  NMR, Nuclear magnetic resonance, Nuclear Overhauser effect, Simulated annealing, Distance geometry, Threedimensional solution structure, Symmetric oligomer

Abbreviations:  ArgRN, Arg Repressor N-terminal domain, dsRBD, double-stranded RNA binding domain, PH, Pleckstrin homology, KH, K homology, PDI, Protein disulphide isomeraze, ssDBP, single stranded DNA binding protein, MDSA, molecular dynamics-based simulated annealing, SA, simulated annealing, RMSD, root mean square difference, RD, reliability distance, α,β,γ,σ, coefficients of the soft-square potential, δppm, frequency tolerance, Δ+,Δ, error estimates for NOE derived distance, d,dif, distance between atoms i and j in a structural model, dmin, minimum distance in an ensemble of structures, dave, average distance in an ensemble of structures, , summed distance, D, distance (single or summed), E, energy, kNOE, NOE energy constant, L, lower limit on distance, Nδ, number of possible assignments within δppm, Sconv, number of converged structures in an ensemble, U, upper limit on distance, V, NOE volume, w, weight

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PII: S0079-6565(97)00025-3

Progress in Nuclear Magnetic Resonance Spectroscopy
Volume 32, Issue 2 , Pages 107-139, 30 April 1998